14.3 Folate

14.3.1 Global tests

Type III ANOVA on main and interaction effects, and LM model summaries.

PRL (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 228.965239 1 <0.0001 ***
Folate 3.359807 1 0.07 ns
Study 1.756550 1 0.2 ns
GA_samp 175.888698 1 <0.0001 ***
Folate:Study 4.059328 1 0.04 *
hPL (μg/ml)
Chisq Df Pr(>Chisq)
(Intercept) 43.0482224 1 <0.0001 ***
Folate 0.1328403 1 0.7 ns
Study 8.9698883 1 0.003 **
GA_samp 2493.5495809 1 <0.0001 ***
Folate:Study 0.0827964 1 0.8 ns
GH2 (ng/ml)
Chisq Df Pr(>Chisq)
(Intercept) 409.0605252 1 <0.0001 ***
Folate 0.6198622 1 0.4 ns
Study 0.0187381 1 0.9 ns
GA_samp 1067.1255134 1 <0.0001 ***
Folate:Study 0.0121091 1 0.9 ns
Insulin (uU/ml)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 4.281 4.281 0.04 *
3 Study 1 Inf 2.539 2.539 0.1 ns
4 GA_samp 1 Inf 0.785 0.785 0.4 ns
2 Folate:Study 1 Inf 2.203 2.203 0.1 ns
HOMA-IR
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 4.835 4.835 0.03 *
3 Study 1 Inf 8.719 8.719 0.003 **
4 GA_samp 1 Inf 0.344 0.344 0.6 ns
2 Folate:Study 1 Inf 2.305 2.305 0.1 ns
Glucose (mmol/L)
model term df1 df2 F.ratio Chisq p.value
1 Folate 1 Inf 1.538 1.538 0.2 ns
3 Study 1 Inf 74.114 74.114 <0.0001 ***
4 GA_samp 1 Inf 0.683 0.683 0.4 ns
2 Folate:Study 1 Inf 0.045 0.045 0.8 ns

14.3.2 Model summary

Ratio of Geometric means (95% CI)

PRL (ng/ml)
Var Effect P
(Intercept) 15.90 (11.12, 22.74) <0.0001
Folate 1.00 (1.00, 1.00) 0.07
StudySTOP 0.92 (0.81, 1.04) 0.19
GA_samp 1.15 (1.13, 1.18) <0.0001
Folate:StudySTOP 1.00 (1.00, 1.00) 0.04
hPL (μg/ml)
Var Effect P
(Intercept) 1.86 (1.54, 2.24) <0.0001
Folate 1.00 (1.00, 1.00) 0.82
StudySTOP 1.12 (1.06, 1.18) 0.0001
GA_samp 1.34 (1.32, 1.36) <0.0001
GH2 (ng/ml)
Var Effect P
(Intercept) 0.08 (0.06, 0.10) <0.0001
Folate 1.00 (1.00, 1.00) 0.15
StudySTOP 0.99 (0.92, 1.07) 0.80
GA_samp 1.30 (1.28, 1.32) <0.0001
Insulin (uU/ml)
Var Effect P
(Intercept) 13.38 (8.46, 21.17) <0.0001
Folate 1.00 (1.00, 1.00) 0.008
StudySTOP 0.80 (0.69, 0.92) 0.002
GA_samp 1.01 (0.98, 1.04) 0.36
HOMA-IR
Var Effect P
(Intercept) 3.45 (2.13, 5.60) <0.0001
Folate 1.00 (1.00, 1.00) 0.005
StudySTOP 0.69 (0.59, 0.80) <0.0001
GA_samp 1.01 (0.98, 1.04) 0.53
Glucose (mmol/L)
Var Effect P
(Intercept) 5.72 (5.23, 6.26) <0.0001
Folate 1.00 (1.00, 1.00) 0.19
StudySTOP 0.86 (0.84, 0.88) <0.0001
GA_samp 1.00 (0.99, 1.00) 0.41

14.3.3 Estimated means

Estimated marginal means (or adjusted means) adjusted for GA at sampling.

Results are averaged over the levels of Serum folate concentration (nM) and may be misleading when interaction term is involved.

PRL (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 95.30925 7.192089 88.93194 82.03834 110.7269
STOP 97.23356 6.934238 71.07960 84.34538 112.0911
hPL (μg/ml)
Study response SE df lower.CL upper.CL
SCOPE 88.35763 2.342321 149.1199 83.84834 93.10943
STOP 98.75798 2.393632 104.5450 94.12386 103.62026
GH2 (ng/ml)
Study response SE df lower.CL upper.CL
SCOPE 2.316474 0.0647714 411.4319 2.192586 2.447362
STOP 2.294230 0.0533669 229.2031 2.191451 2.401829
Insulin (uU/ml)
Study response SE df asymp.LCL asymp.UCL
SCOPE 14.61989 0.7155733 Inf 13.28256 16.09186
STOP 11.67234 0.4284489 Inf 10.86209 12.54302
HOMA-IR
Study response SE df asymp.LCL asymp.UCL
SCOPE 3.542497 0.1840592 Inf 3.199508 3.922254
STOP 2.437793 0.0945271 Inf 2.259389 2.630285
Glucose (mmol/L)
Study response SE df asymp.LCL asymp.UCL
SCOPE 5.484230 0.050988 Inf 5.385200 5.585080
STOP 4.720143 0.034892 Inf 4.652249 4.789028

14.3.4 Comparisons

Effect for every 50 unit increase in Serum folate concentration (nM).
Hormone Contrast Study Ratio of Geometric means (95% CI) P-value
PRL (ng/ml) Folate89.2 / Folate39.2 SCOPE 0.94 (0.88, 1.00) 0.07
PRL (ng/ml) Folate89.2 / Folate39.2 STOP 1.02 (0.97, 1.07) 0.4
hPL (μg/ml) Folate89.2 / Folate39.2 SCOPE 1.00 (0.98, 1.02) 0.8
hPL (μg/ml) Folate89.2 / Folate39.2 STOP 1.00 (0.98, 1.02) 0.8
GH2 (ng/ml) Folate89.2 / Folate39.2 SCOPE 0.98 (0.95, 1.01) 0.1
GH2 (ng/ml) Folate89.2 / Folate39.2 STOP 0.98 (0.95, 1.01) 0.1
Insulin (uU/ml) Folate89.2 / Folate39.2 SCOPE 0.93 (0.88, 0.98) 0.008
Insulin (uU/ml) Folate89.2 / Folate39.2 STOP 0.93 (0.88, 0.98) 0.008
HOMA-IR Folate89.2 / Folate39.2 SCOPE 0.92 (0.87, 0.98) 0.005
HOMA-IR Folate89.2 / Folate39.2 STOP 0.92 (0.87, 0.98) 0.005
Glucose (mmol/L) Folate89.2 / Folate39.2 SCOPE 0.99 (0.98, 1.00) 0.2
Glucose (mmol/L) Folate89.2 / Folate39.2 STOP 0.99 (0.98, 1.00) 0.2

14.3.5 Diagnostics

Model diagnositcs.

Diagnositcs for PRL (ng/ml)

Diagnositcs for hPL (μg/ml)

Diagnositcs for GH2 (ng/ml)

Diagnositcs for Insulin (uU/ml)

Diagnositcs for HOMA-IR

Diagnositcs for Glucose (mmol/L)

14.3.6 Plots